Adaptive-Optical 3D Microscopy For Microfluidic Multiphase Flows
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Adaptive-Optical 3D Microscopy For Microfluidic Multiphase Flows

09/09/2024 TranSpread

Imaging and measurements based on optical microscopy can be severely impaired if the access exhibits variations of the refractive index. In the case of flow measurements through fluctuating liquid-gas boundaries, refraction introduces dynamical aberrations that increase the measurement uncertainty. This is prevalent at multiphase flows (e. g. droplets, film flows) that occur in many technical applications as for example in coating or cleaning processes and the water management in fuel cells. In this paper (doi: https://doi.org/10.37188/lam.2024.037), we present a novel approach based on adaptive optics for correcting the dynamical aberrations in real time and thus reducing the measurement uncertainty. The shape of the fluctuating water-air interface is sampled with a reflecting light beam (Fresnel Guide Star) and a Hartmann-Shack wavefront sensor which makes it possible to correct its influence with a deformable mirror in closed-loop operation. Three-dimensional flow measurements are achieved by using a double-helix point spread function. We measure the flow inside a sessile, oscillating 50-µl droplet on an opaque gas diffusion layer for fuel cells and show that the temporally varying refraction at the droplet surface causes a systematic underestimation of the flow field magnitude corresponding to the first droplet eigenmode which plays a major role in their detachment mechanism. We demonstrate that the adaptive optics correction is able to reduce this systematic error. The adaptive optics system can pave the way to a deeper understanding of water droplet formation and detachment which can help to improve the efficiency of fuels cells.

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References

DOI

10.37188/lam.2024.037

Original Source URL

https://doi.org/10.37188/lam.2024.037

Funding information

The research project IGF-Nr. 21190 BG/2 from the research association DECHEMA e. V. is supported by the Federal Ministry of Economic Affairs and Energy through the German Federation of Industrial Research Associations (AiF) as part of the programme for promoting industrial cooperative research (IGF) on the basis of a decision by the German Bundestag.
Furthermore, this work is partially supported by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) – project number 459505672.

About Light: Advanced Manufacturing (LAM)

Light: Advanced Manufacturing (LAM) is a new, highly selective, open-access, and free of charge international sister journal of the Nature Journal Light: Science & Applications. The journal aims to publish innovative research in all modern areas of preferred light-based manufacturing, including fundamental and applied research as well as industrial innovations.

https://doi.org/10.37188/lam.2024.037
Paper title: Adaptive-optical 3D microscopy for microfluidic multiphase flows
Attached files
  • Simplified overview of the optical setup. The rays of the fluorescence particle are refracted by the fluctuating water-air interface. Because this aberrating layer can be considered planar, the correction performance can be increased by placing the deformable mirror in a conjugate plane to the water-air interface. The height profile of the water surface is sampled with a probe beam (green), which we term Fresnel Guide Star (FGS), and a wavefront sensor. The probe beam (FGS) is coupled into the system via a beam splitter and its reflex at the water-air interface is imaged to the deformable mirror and the wavefront sensor. For the sake of simplicity, the 3D localisation microscope is illustrated here as 2D microscope.
  • Example power density spectrum of the apparent motion of a particle resting on the substrate. Without correction, the particle position seems to oscillate because of the light refraction at the fluctuating water-air interface. With correction, this virtual motion (measurement error) is significantly suppressed. The horizontal axis is the frequency in Hertz.
09/09/2024 TranSpread
Regions: North America, United States, Europe, Germany
Keywords: Business, Manufacturing

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