Plastic waste pollution represents a critical environmental challenge with increasing implications for the well-being and health of future generations. Food packaging is a major source of micro and nanoplastic (MNPLs) contamination and inhalation and ingestion is the main route of human exposure.
A study by the Mutagenesis Group of the UAB Department of Genetics and Microbiology has successfully obtained and characterised micro and nanoplastics derived from several types of commercially available tea bags. The UAB researchers observed that when these tea bags are used to prepare an infusion, huge amounts of nano-sized particles and nanofilamentous structures are released, which is an important source of exposure to MNPLs.
The tea bags used for the research were made from the polymers nylon-6, polypropylene and cellulose. The study shows that, when brewing tea, polypropylene releases approximately 1.2 billion particles per milliliter, with an average size of 136.7 nanometres; cellulose releases about 135 million particles per milliliter, with an average size of 244 nanometres; while nylon-6 releases 8.18 million particles per milliliter, with an average size of 138.4 nanometres.
To characterise the different types of particles present in the infusion, a set of advanced analytical techniques such as scanning electron microscopy (SEM), transmission electron microscopy (TEM), infrared spectroscopy (ATR-FTIR), dynamic light scattering (DLS), laser Doppler velocimetry (LDV), and nanoparticle tracking analysis (NTA) were used. “We have managed to innovatively characterise these pollutants with a set of cutting-edge techniques, which is a very important tool to advance research on their possible impacts on human health,” remarks UAB researcher Alba Garcia.
Interactions with human cells observed for the first time
The particles were stained and exposed for the first time to different types of human intestinal cells to assess their interaction and possible cellular internalisation. The biological interaction experiments showed that mucus-producing intestinal cells had the highest uptake of micro and nanoplastics, with the particles even entering the cell nucleus that houses the genetic material. The result suggests a key role for intestinal mucus in the uptake of these pollutant particles and underscores the need for further research into the effects that chronic exposure can have on human health.
“It is critical to develop standardised test methods to assess MNPLs contamination released from plastic food contact materials and to formulate regulatory policies to effectively mitigate and minimise this contamination. As the use of plastic in food packaging continues to increase, it is vital to address MNPLs contamination to ensure food safety and protect public health”, researchers add.
The study was developed under the framework of the European project PlasticHeal (https://www.plasticheal.eu/en), coordinated by, Alba Hernández lecturer in the Department of Genetics and Microbiology at the UAB. Researchers from the UAB Mutagenesis Group Alba García-Rodríguez, Ricard Marcos and Gooya Banaei, first author of the research article, were also involved in the study, with the collaboration of researchers from the Helmholtz Centre for Environmental Research in Leipzig, Germany.