New research offers hope for preventing epilepsy after traumatic brain injury
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New research offers hope for preventing epilepsy after traumatic brain injury


Monday 27 January 2025: A new international study has unveiled critical insights in understanding post-traumatic epilepsy (PTE), a condition that can develop following traumatic brain injury. Led by researchers at FutureNeuro, the Research Ireland Centre for Translational Brain Science and RCSI University of Medicine and Health Sciences and published in Theranostics, the study highlights the important role played by a receptor in the brain called P2X7. It suggests how we could both reduce epilepsy risk and predict which patients are most at risk of developing PTE by targeting this receptor.
Traumatic brain injury (TBI), caused by physical trauma to the head, is one of the leading causes of long-term disability and death worldwide. PTE is a common outcome, characterised by recurring seizures that profoundly impact quality of life. At the moment, up to 30% of PTE patients do not respond to existing medications, and no treatments are currently available to predict or prevent the development of epilepsy following traumatic brain injury.
The collaborative research, led by FutureNeuro and RCSI, involving institutions including Trinity College Dublin, CIC biomaGUNE, Soochow University, and the Institute for Stroke and Dementia Research, identifies the P2X7 receptor as a key driver of abnormal brain activity after brain injury. In preclinical models, blocking this receptor shortly after injury significantly reduced brain hyperexcitability, minimised brain damage, and improved behaviour, underscoring its promise as a therapeutic target for preventing epilepsy. 
By looking at the activity of the P2X7 receptor using a PET scan, the authors also suggest a potential new diagnostic tool. The uptake by the brain of a specialised P2X7 receptor tracer shortly after injury was found to correlate with seizure risk weeks later. This tool could help clinicians identify at-risk patients early, enabling timely and tailored interventions. 
Dr Tobias Engel, FutureNeuro Investigator and Senior Lecturer in the RCSI Department of Physiology and Medical Physics, commented: "Traumatic brain injury is a major cause of epilepsy in adults, with many patients unable to benefit from existing anti-seizure treatments. Our research has identified the P2X7 receptor as a promising new target, offering the potential to prevent epilepsy before it develops, sparing patients from seizures and the burdens of ongoing medication." 
“While additional research is required to confirm our findings and explore their application in clinical settings, we’ve made a significant step forward in addressing the unmet need for early intervention in post-traumatic epilepsy” added Dr David Loane, Associate Professor in Neuroscience at Trinity College Dublin. “This was made possible through extensive multidisciplinary collaboration, demonstrating the power of shared expertise in advancing epilepsy research.”
Dr Jordi Llop, Principal Investigator at CIC biomaGUNE said: “By identifying a potential therapeutic target and a corresponding predictive diagnostic tool, this research opens new avenues for personalised care, improved outcomes and a better quality of life for patients with traumatic brain injury at risk of epilepsy.”
The study involved extensive multidisciplinary collaboration, including key industry partners, Janssen and Affectis Pharmaceuticals. Funded by Research Ireland, the project leveraged diverse expertise to tackle the complex challenges of traumatic brain injury and epilepsy.
ENDS
Theranostics 2025; 15(4):1399-1419. doi:10.7150/thno.97254

Research Paper
P2X7R antagonism suppresses long-lasting brain hyperexcitability following traumatic brain injury in mice

Mariana Alves1#, Laura de Diego-Garcia1,2#, Gloria Vegliante3, Oscar Moreno4, Beatriz Gil1, Pedro Ramos-Cabrer4,5, Meghma Mitra1, Ana Fernandez Martin1, Aida Menéndez-Méndez1,6, Yitao Wang1,7, Nathan Ryzewski Strogulski3, Meng-Juan Sun1, Ciara Melia1, Giorgia Conte1, Sandra Plaza-García4, Igor Khalin8,9,10, Xinchen Teng7, Nikolaus Plesnila8,9, Bert Klebl11, Klaus Dinkel12, Michael Hamacher13, Anindya Bhattacharya14, Marc Ceusters15,16, James Palmer14, David J. Loane3, Jordi Llop4, David C. Henshall1,17, Tobias Engel1,17 Corresponding address

1. Department of Physiology & Medical Physics, RCSI University of Medicine & Health Sciences, Dublin D02 YN77, Ireland.
2. Department of Optometry, Faculty of Optics and Optometry, Universidad Complutense de Madrid, Avda. Arcos de Jalon 118, 28040 Madrid, Spain.
3. School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College, Dublin, Ireland.
4. CIC biomaGUNE, Basque research and Technology Alliance (BRTA), P° Miramon 182, 20014 San Sebastian, Gipuzkoa, Spain.
5. Ikerbasque Basque Foundation for Science, Bilbao, Spain.
6. Department of Medicine, Faculty of Biomedical Sciences and Health, Universidad Europea de Madrid, C. Tajo, s/n, 28670 Villaviciosa de Odón, Madrid, Spain.
7. College of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu 215123, China.
8. Institute for Stroke and Dementia Research (ISD), LMU University Hospital, Ludwig-Maximilians-University (LMU) Munich, 81377 Munich, Germany.
9. Munich Cluster for Systems Neurology (SyNergy), 81377 Munich, Germany.
10. Normandie University, UNICAEN, INSERM UMR-S U1237, Physiopathology and Imaging of Neurological Disorders (PhIND), GIP Cyceron, Institute Blood and Brain @ Caen-Normandie (BB@C), Caen, France.
11. KHAN Technology Transfer Fund I GmbH & Co. KG, Otto-Hahn-Straße 15, 44227 Dortmund, Germany.
12. Lead Discovery Center GmbH, Otto-Hahn-Straße 15, 44227 Dortmund, Germany.
13. Affectis Pharmaceuticals AG, Otto-Hahn-Straße 15, 44227 Dortmund, Germany.
14. Janssen Research and Development LLC, San Diego, California, USA.
15. Janssen Pharmaceutica NV, Beerse, Belgium.
16. The Marc Ceusters Company, BV, Diest, Belgium.
17. FutureNeuro Research Ireland Centre for Translational Brain Science, RCSI University of Medicine and Health Sciences, Dublin D02 YN77, Ireland.
# Authors contributed equally.

✉ Corresponding author: Tobias Engel, Ph.D., Department of Physiology & Medical Physics, Royal College of Surgeons in Ireland, University of Medicine and Health Sciences, Dublin
Regions: Europe, Ireland, Belgium, Germany, Asia, China, North America, United States
Keywords: Health, Medical, Science, Life Sciences

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