Apoptotic Vesicles Loaded Fibrous Scaffolds Promote Wound Healing
en-GBde-DEes-ESfr-FR

Apoptotic Vesicles Loaded Fibrous Scaffolds Promote Wound Healing

06/03/2025 Compuscript Ltd
A recent study showed that paracrine delivery of miR-21a-5p by bone marrow mesenchymal stem cells-derived apoptotic bodies (BMSC-derived AB)-loaded polycaprolactone (PCL) scaffolds promote wound healing by regulating macrophage polarization to M2 phenotype.

Macrophages are critical players mediating wound healing, and the microenvironment of the wound influences macrophage polarization to either the M1 pro-inflammatory phenotype in the early stages or the M2 pro-healing phenotype in the later stages. Dysfunction of the macrophage polarization M2 phenotype, lower macrophage numbers, and reduced anti-inflammatory and angiogenic capacity are causatives of long-term non-healing.

Mesenchymal stem cells (MSCs) aid tissue repair and regeneration by delivering endogenous regulatory factors via paracrine extracellular vesicles. Previous studies have shown that BMSC-derived ABs induce macrophage polarization to the M2 phenotype, which mitigates inflammation and enhances the migration and proliferation of fibroblasts.

In this study, published in the Genes and Diseases journal, researchers at Chongqing Medical University and Sichuan Provincial Orthopedic Hospital investigate the wound healing efficacy and macrophage polarization potential of electrospun PCL scaffolds loaded with BMSC-ABs, in vitro and in vivo.

The results showed that the BMSC-AB loaded PCL scaffolds could prevent or reduce delayed wound healing by reprogramming the macrophages into the M2 phenotype and reducing inflammatory infiltration, thereby potentiating anti-inflammatory and angiogenic effects. Furthermore, the paracrine delivery of miR-21a-5p through the slow-release of BMSC-AB by the PCL scaffolds reprograms macrophages to the M2 phenotype by regulating the expression of the CCL-1 gene, promotes fibroblast migration, and initiates angiogenesis by secreting anti-inflammatory cytokines and the angiogenesis-related factors, VEGF and vWF.

In conclusion, the BMSC-AB-loaded PCL scaffolds, through the targeted and sustained delivery of miR-21a-5p, drive macrophage polarization to the M2 phenotype, which exerts a dual effect by regulating inflammation and angiogenesis, thereby synergistically promoting wound healing.

Reference

Title of the original paper: Fibrous scaffolds loaded with BMSC-derived apoptotic vesicles promote wound healing by inducing macrophage polarization.

Journal: Genes & Diseases
Genes & Diseases is a journal for molecular and translational medicine. The journal primarily focuses on publishing investigations on the molecular bases and experimental therapeutics of human diseases. Publication formats include full length research article, review article, short communication, correspondence, perspectives, commentary, views on news, and research watch.

DOI: https://doi.org/10.1016/j.gendis.2024.101388

Funding Information:

National Natural Science Foundation of China (No. 82072443, 82372425)
The Chongqing Outstanding Project of Overseas Chinese Entrepreneurship and Innovation Support Program (China) (No. CX2022032)
The Articular Cartilage Tissue Engineering and Regenerative Medicine Team, Chongqing Medical University (No. W0080)
The General Project of Natural Science Foundation of Chongqing, China (No. CSTB2023NSCQ-MSX0166)
The “Tomorrow Cup” Teacher‒Student Cocreation Teaching and Research Innovation Project of International Medical College of Chongqing Medical University (No. KY20220204)
The Chengdu Medical Research Project (Sichuan, China) (No. 2023191)
The China Postdoctoral Science Foundation (No. 2022M710557)
The Natural Science Foundation of Chongqing, China (No. CSTB2023NSCQ-BHX0011)
The Young Excellent Science and Technology Talent Project of the First Affiliated Hospital of Chongqing Medical University (No. ZYRC2022-05)

# # # # # #

Genes & Diseases publishes rigorously peer-reviewed and high quality original articles and authoritative reviews that focus on the molecular bases of human diseases. Emphasis is placed on hypothesis-driven, mechanistic studies relevant to pathogenesis and/or experimental therapeutics of human diseases. The journal has worldwide authorship, and a broad scope in basic and translational biomedical research of molecular biology, molecular genetics, and cell biology, including but not limited to cell proliferation and apoptosis, signal transduction, stem cell biology, developmental biology, gene regulation and epigenetics, cancer biology, immunity and infection, neuroscience, disease-specific animal models, gene and cell-based therapies, and regenerative medicine.

Scopus CiteScore: 7.3 | Impact Factor: 6.9

# # # # # #

More information: https://www.keaipublishing.com/en/journals/genes-and-diseases/

Editorial Board: https://www.keaipublishing.com/en/journals/genes-and-diseases/editorial-board/

All issues and articles in press are available online in ScienceDirect (https://www.sciencedirect.com/journal/genes-and-diseases).

Submissions to Genes & Disease may be made using Editorial Manager (https://www.editorialmanager.com/gendis/default.aspx ).

Print ISSN: 2352-4820
eISSN: 2352-3042
CN: 50-1221/R
Contact Us: editor@genesndiseases.com

X (formerly Twitter): @GenesNDiseases (https://x.com/GenesNDiseases)
https://tinyurl.com/he2d9fvb
Attached files
  • (A) Immunostaining of 0–100 μg/mL PCL-BMSC-ABs incubated for 4 h. Green: cytoplasm; blue: nucleus; red: ABs. (B) Immunostaining of 0–100 μg/mL PCL-ABs incubated for 6 h. Green: cytoplasm; blue: nucleus; red: ABs. (C) Relative fluorescence intensity of 0–100 μg/mL PCL-BMSC-ABs incubated for 4 h and 6 h n = 3; ∗∗P < 0.01. (D) RAW264.7 cell viability after incubation with PCL-ABs. (E) Western blot analysis of Mϕs reprogrammed with the M2 phenotype treated with 50 μg/mL PCL-ABs. (F) Relative grayscale values of western blot analysis of 50 μg/mL PCL-BMSC-ABs Mϕs reprogrammed with the M2 phenotype. n = 3; ∗∗P < 0.01, ∗P < 0.05. (G) Flow cytometry comparison of CD206-positive M1 Mϕs and Mϕs incubated with 50 μg/mL PCL-BMSC-ABs for different periods.
  • (A) The top 50 known miRNAs detected in BMSC-ABs. (B) miRNA-mRNA regulatory network. (C) The binding of miR-21a-5p to the target gene CCL-1 in 293T cells validated by dual luciferase assay. (D) Colocalization of miR-21a-5p with Mϕs. (E) Fluorescence intensity analysis. n = 3; ∗P < 0.05. (F) Western blot analysis of the effect of the miR-21a-5p inhibitor on the reprogramming of BMSC-ABs to Mϕs. (G) Relative grayscale values of the western blot analysis of miR-21a-5p inhibitor's effect on the reprogramming of BMSC-ABs that drive Mϕs to M2-Mϕs. n = 3; ∗∗∗P < 0.001. (H) Quantitative PCR analysis of miR-21a-5p inhibitor's effect on the reprogramming of BMSC-AB-driven Mϕs to M2-Mϕs. n = 3; ∗∗∗P < 0.001.
  • (A) Acquisition of BMSC-ABs. (B) Identification of apoptotic cells by flow cytometry. (C) Morphological images of ABs. Left: scanning electron microscopy (SEM) images of ABs (scale bar, 2 μm); right: particle size of ABs measured by dynamic light scattering (DLS). (D) Zeta potential of the ABs. (E) Flow chart of PCL scaffolds prepared by electrostatic filament mimicking technique. (F) SEM image of an electrostatic filament mimicking the PCL scaffold. Scale bar, 20 μm. (G) SEM images of electrostatic filament-mimicking PCL scaffolds loaded with ABs. Red arrows indicate sites representing ABs. (H) Identification of ABs by western blot. (I) Release rate of AB-loaded PCL scaffolds.
06/03/2025 Compuscript Ltd
Regions: Europe, Ireland, Asia, China
Keywords: Science, Life Sciences, Business, Medical & pharmaceutical

Disclaimer: AlphaGalileo is not responsible for the accuracy of content posted to AlphaGalileo by contributing institutions or for the use of any information through the AlphaGalileo system.

Testimonials

For well over a decade, in my capacity as a researcher, broadcaster, and producer, I have relied heavily on Alphagalileo.
All of my work trips have been planned around stories that I've found on this site.
The under embargo section allows us to plan ahead and the news releases enable us to find key experts.
Going through the tailored daily updates is the best way to start the day. It's such a critical service for me and many of my colleagues.
Koula Bouloukos, Senior manager, Editorial & Production Underknown
We have used AlphaGalileo since its foundation but frankly we need it more than ever now to ensure our research news is heard across Europe, Asia and North America. As one of the UK’s leading research universities we want to continue to work with other outstanding researchers in Europe. AlphaGalileo helps us to continue to bring our research story to them and the rest of the world.
Peter Dunn, Director of Press and Media Relations at the University of Warwick
AlphaGalileo has helped us more than double our reach at SciDev.Net. The service has enabled our journalists around the world to reach the mainstream media with articles about the impact of science on people in low- and middle-income countries, leading to big increases in the number of SciDev.Net articles that have been republished.
Ben Deighton, SciDevNet

We Work Closely With...

  • BBC
  • The Times
  • National Geographic
  • University of Cambridge
  • iesResearch
Copyright 2025 by AlphaGalileo Terms Of Use Privacy Statement