Leaf color is more than just an aesthetic trait—it is a vital agronomic characteristic that directly influences photosynthetic efficiency and crop yield. Variations in leaf pigmentation often stem from disrupted chloroplast function and impaired chlorophyll synthesis. While previous studies have highlighted the role of PPR proteins in chloroplast RNA editing and development, the precise mechanisms governing leaf color variation in cabbage remain largely unexplored. This knowledge gap has driven researchers to delve deeper into the genetic and molecular foundations of leaf pigmentation in cabbage.
On January 10, 2024, a study (DOI: 10.1093/hr/uhae006) published in Horticulture Research by scientists from Nanjing Agricultural University and the Chinese Academy of Agricultural Sciences unveiled the pivotal role of the BoYgl-2 gene in cabbage leaf color formation. The research focused on a naturally occurring yellow-green leaf mutant and provided a comprehensive genetic and molecular analysis to uncover the underlying mechanisms of this distinct phenotype.
The study identified and characterized a unique yellow-green leaf mutant (4036Y) in cabbage, exhibiting significantly reduced chlorophyll levels and abnormal chloroplast development during early leaf growth. Genetic analysis revealed that the trait is governed by a single recessive gene, BoYgl-2, which encodes a P-type PPR protein. Through fine mapping and sequencing, researchers pinpointed a 162-kb chromosomal deletion in the BoYgl-2 locus of the mutant. Functional complementation experiments confirmed the gene's role—reintroducing BoYgl-2 from normal-green leaf cabbage (4036G) successfully restored the wild-type phenotype, reinforcing its essential function in chloroplast development.
Further analysis of RNA editing revealed that in the mutant, the C-to-U editing efficiency of several crucial chloroplast genes—atpF, rps14, petL, and ndhD—was significantly compromised. However, in BoYgl-2 overexpression lines, the editing efficiency of these genes was restored to near-normal levels, mirroring those found in 4036G. Additionally, gene expression profiling showed substantial alterations in chloroplast-related pathways, with key genes involved in chloroplast development and chlorophyll biosynthesis notably downregulated. These findings provide a molecular blueprint for understanding how BoYgl-2 regulates chloroplast RNA editing and chlorophyll biosynthesis, offering new insights into the genetic architecture of leaf color formation in cabbage.
Dr. Xilin Hou, one of the study's corresponding authors, emphasized the significance of these findings, stating, "Our research not only deepens our understanding of the genetic and molecular mechanisms behind leaf color formation in cabbage but also opens new possibilities for crop improvement through targeted genetic interventions."
The discovery of BoYgl-2 and its role in chloroplast RNA editing carries profound implications for agricultural breeding. By developing precise genetic markers for BoYgl-2, plant breeders can streamline the selection process for desirable leaf color traits in cabbage and potentially other crops. This marker-assisted selection approach could accelerate breeding efforts, leading to enhanced crop varieties with optimized agronomic traits. Moreover, a deeper understanding of chloroplast RNA editing and chlorophyll biosynthesis unlocks new opportunities for genetic modifications aimed at improving photosynthetic efficiency and boosting crop yields. This research not only advances fundamental plant biology but also contributes to sustainable agriculture and global food security.
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References
DOI
10.1093/hr/uhae006
Original Source URL
https://doi.org/10.1093/hr/uhae006
Funding information
This work was supported by a grant from the National Natural Science Foundation of China (31872948).
About Horticulture Research
Horticulture Research is an open access journal of Nanjing Agricultural University and ranked number one in the Horticulture category of the Journal Citation Reports ™ from Clarivate, 2022. The journal is committed to publishing original research articles, reviews, perspectives, comments, correspondence articles and letters to the editor related to all major horticultural plants and disciplines, including biotechnology, breeding, cellular and molecular biology, evolution, genetics, inter-species interactions, physiology, and the origination and domestication of crops.